Evaluation of the smartcycler ii system for realtime detection of. Find and purchase cepheid smartcycler products at invitrogen life science technologies. Rapid and sensitive detection of mycobacterium dna using. Information on this page may be partial or incomplete and may contain mistakes or inaccurate information. Additionally, by uploading or otherwise transmitting material to any area of the web site you.
Evaluation of the smartcycler ii system for realtime. The companys systems enable rapid, sophisticated molecular testing for organisms and geneticbased diseases. Cepheid s is staffed with experienced technical support specialists and scientists. Michael t taylor, william mcmillan, rapid and sensitive detection of mycobacterium dna using cepheid smartcycler. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. Cepheid launches ce mark certification smart cmv in vitro diagnostic test for smartcycler pcr platform. Cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. The detection of microbial dna in clinical samples by nucleic acid amplification methods is often limited by the low concentration of organisms present in the s. Toxoplasmosis in a woodchuck marmota monax and two. Maxima probe qpcr master mix, rox solution provided. Cepheid smartcycler sc1600 starter system, desktop medex.
For further questions please contact cepheid in the foyer. Cepheid smart gbs test for group b streptococcus, run on. Thermal cyclers cepheids smart cycler thermal cycler system monitors the accumulation of pcr products by performing both amplification and immediate. Acros organics, fisher scientific, inverting the tube 30 to 40. Designing science equipment for the international space station and beyond.
Realtime pcr detection of shigatoxin producing escherichia coli stec, serotype o157 and non o157 in fresh produce and food with nono157 isolation and identification revision. Csu surplus property is selling a cepheid smart cycler ii td realtime pcr system previously used in a university setting. Smartcycler ii the smartcycler is a realtime pcr instrument with 16, 34, 48, 64, 80 or 96 independent reaction sites. If you require additional information or technical assistance, please feel free to email our technical support group at. Smartcycler icore module independently programmable unit. For the smart cycler instrument, data acquisition and analysis were done by using cepheid smart cycler software version 1. Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the. Txrd and cy5 channels smart cycler operator manual. The smartcycler system at the heart of the smartcycler system is the icore module a reliable, solidstate optical system with no moving parts. Detection of food pathogens using the smart cycler ii gene. If you require additional information or technical assistance, please feel free to. The reagents for the amplification reaction on the cepheid smartcycler were provided in the euroclone duplica real time dual easy ct assay.
Cepheid launches ce mark certification smart ebvtm in vitro diagnostic test for smartcyclerr platform read this article along with other careers information, tips and advice on biospace. The smart cycler software ensures ease of use for the sixteen independently controlled icore modules found on the smart cycler processing block. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. Cepheid inc is an american molecular diagnostics company, now part of danaher, that develops, manufactures and markets fully integrated systems for testing in the clinical market, and for application in its original nonclinical market. For the smart cycler, data were analyzed by using the cepheid smart cycler software version 1. Cepheid smartcycler ii td pcr manufacturer specifications. Cepheid smart gbs test for group b streptococcus, run on the smartcyclerr system, receives 510k fda clearance read this article along with other careers information, tips and advice on biospace. Description of the cepheid smartcycler starter system, desktop. The smart cycler settings consisted of a primary curve analysis with a manual threshold setting of 10, background subtraction turned on, boxcar average set to five cycles, background minimum cycle set to 5, and background maximum cycle set to 45. Cepheid warrants that the smartcycler processing block the instrument i is free from. Pdf evaluation of the portable cepheid smartcycler real. Smartcycler sc1600 starter system includes a processing block, software, operators manual, accessory kit, and desktop computer with cdrw drive and flatpanel monitor. Our cepheid trained staff is available for onsite customer training for all cepheid. Our it staff was able to power on the smart cycler and included laptop.
Concentration, extraction, and direction of norovirus and. Method 1611 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal rna gene lsrrna, 23s rrna from these organisms. Amplification and detection on the cepheid smart cycler. Data acquisition and analysis were done with cepheid smart cycler software version 2. Evaluation of the portable cepheid smartcycler realtime. Cepheid launches ce mark certification smart ebvtm in. Cepheid smart cycler 9000330 automated realtime pcr. Medwow disclaims any obligation, responsibility or liability which concerns or relates to any information.
If further optimization is needed, determine the optimal concentration within the range of 0. Cepheid smartcycler ii thermal cycler automated realtime. Cepheid smartcycler system software license agreement license grant. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. United states department of agriculture agricultural. Cepheid launches ce mark certification smart cmv in vitro. Smallpox and panorthopox virus detection by realtime 3.
However, there are several obstacles to clinical viability, including time required for testing, cost, and interpretation of. You are also free to make these changing later on while. Objectiveto compare the smartcycler ii system cepheid, sunnyvale, calif. Method 1609 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal ribonucleic. Post a free request for proposal for a cepheid smartcycler ii thermal cycler automated realtime pcr system and get quotes from our gold service dealers. Cepheid enabling access to molecular diagnostic testing. The staff is available 12 hours a day, via toll free number, by email, or through the cepheid web site.
Each of the standard 16 icore modules expandable to 96 requires no warmup time, no routine maintenance, and no normalization dye. The smartcycler systems optics allow fluorescence to be measured in four channels at the same time. Thermal cycling for the smart cycler instrument was performed as follows. I agree that cepheid shall have no obligation to pay me or otherwise compensate me in any way for receiving and evaluating the submission, and that any decision by cepheid to partner, purchase or license the submission or any intellectual property associated. Cepheid genotyping is becoming more important in many clinical settings for disease diagnosis and optimized treatment choices. The test can provide a highly accurate result in as little as 30 minutes, during the critical period when detection of gbs may potentially reduce the number of infections and deaths to. Thermal cycler spectral calibration instructions why is spectral calibration necessary. This is a 510k application for a new qualitative realtime polymerase chain reaction pcr assay used with the cepheid smartcycler ii instrument for the in vitro qualitative. Type ii cepheids are variable stars which pulsate with periods typically between 1 and 50 days.
Computer is windows 2000 with cepheid smart cycler version 2. Laboratory considerations for use of cepheid xpert mtbrif. Pdf the ability of the portable cepheid smartcycler realtime pcr. Cepheid accessory for smartcycler pcr systems, cepheid.
Details about cepheid smart cycler 9000330 automated realtime pcr system. Each reaction site can be programmed and run independently. Upon completion of run, transfer purified nucleic acid to nucleasefree tubes. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Cepheid smartcycler system software license agreement. Please refer to the smartcycler operator manual, appendix b, and the. Pdf evaluation of the portable cepheid smartcycler realtime. Preparation of a master mix, which includes all reaction components except template dna, helps to avoid pipetting errors and is an essential step in realtime pcr. Cepheid smart cycler ii td realtime pcr system govdeals. Genomic dna up to 500 ng and plasmid dna up to 10 ng can be used in qpcr with maxima probe qpcr master mix, no rox. Used cepheid smartcycler ii thermal cycler automated real. Cepheids smart cycler instrument facilitates the speed and accuracy of the idistrep b test.
Cepheid smartcycler automated realtime pcr system thermal cycler 16 site. Unlike other intelligence solutions, bciq exclusively supports the unique needs of the biopharma industry and. Manufacturer specifications smartcycler ii td, cepheid. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing. In the thresh setting column, enter manual threshold for each channel influenza a, influenza. Cepheid smart cycler sample processing module extraction and purification of rna or dna in microgravity glovebag to reduce risk for cross contamination with nonsample rna or dna. For the biopharma industry investment, business development and competitive intelligence professionals who require information to support financing, partnering and licensing activities, bciq provides accurate information and context to support profitable and strategic decision making. The cepheid smart cycler dx system is an integrated nucleic acid amplification and detection instrument system based on the proprietary microprocessorcontrolled icore module. Dnasernase free water applied biosystems molecular grade. Objective to compare the smartcycler ii system cepheid, sunnyvale, calif results with those of standard cell culture, to compare the smartcycler ii system results with those of a dedicated polymerase chain reaction facility, and to establish the smartcycler ii system as a polymerase chain reaction method for detecting viral and chlamydial dna from ocular specimens. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory.
The laptop connects to the cycler and recognizes it. During pcr cycling, optical signals from two sequence specific fluorescent probes within the reaction are monitored in real time. Cepheid smart cycler 9000330 automated realtime pcr system. We designed, optimized, and extensively tested several sensitive and specific realtime pcr assays for rapid detection of both smallpox and panorthopox virus dnas. Business health care industry biotechnology industries product introduction biotechnology industry cancer patients analysis diagnostic equipment medical usage. Evaluation of cepheid smartcycler and euroclone duplicareal time. For full access to this pdf, sign in to an existing account, or.